feeble estrus

Construction and identification of a vector expressing short hairpin RNA targeting annexinⅡgene;

膜联蛋白Ⅱ基因短发夹RNA表达载体的构建和鉴定

The effect of short hairpin RNA on hTERT expression;

短发夹RNA对人端粒酶催化亚基表达的影响

Objective To clone shRNA (short hairpin RNA) recombinant plasmid targeting on COX-2 gene and analyze the nucleic acid sequence of the recombinant plasmid.

目的利用RNA干扰技术,以COX-2为靶基因,构建靶向COX-2基因的短发夹RNA(shRNA)重组表达质粒并进行鉴定分析。

Inhibition of ICAM-1 gene expression in liver cancer cells by shRNA;

短发夹RNA对肝癌细胞细胞间粘附分子-1基因表达的影响

Objectives To investigate nano-carrier G9 PAMAM mediated plasmid containing CTGF-shRNA (pCTGF-shRNA) transfection on connective tissue growth factor (CTGF) expression in mouse peritoneal mesothelial cells (MPMCs).

目的研究G9PAMAM纳米载体介导的结缔组织生长因子(CTGF)短发夹RNA(shRNA)质粒(pCTGF-shRNA)对小鼠腹膜间皮细胞CTGF表达的影响。

The annealed oligonucleotides were inserted into the linearized pSIREN-RetroQ vector to construct the recombinant shRNA plasmid(pSIREN-VEGF-C) and then transformed into E.

方法:根据GenBank中VEGF-C序列,设计、合成靶向VEGF-C基因、编码短发夹RNA的两条寡核苷酸序列,退火后用T4连接酶与线性化的pSIREN载体连接,转化感受态大肠杆菌,筛选阳性克隆,抽提质粒,获得重组pSIREN-VEGF-C质粒;BglⅡ和EcoRⅠ双酶切、测序鉴定。

Objective: To transfect a recombinant short hairpin RNA(shRNA)expression vector targeting Livin gene isoform(BIRC71,BIRC72)into the cervical cancer cell line(Hela cell),in an attempt to observe RNAi-mediated silen- cing on Livin gene and the induction of Hela apoptosis.

目的:应用构建成功的Livin异构体(BIRC71,BIRC72)短发夹RNA(short hairpin HNA,shRNA)真核表达载体转染Hela细胞,探讨其对Livin基因的沉默效应及诱导Hela细胞凋亡的作用。

Objective To investigated the effect of short hairpin RNA(shRNA) targeting hypoxia inducible-1α(HIF-1α) on the human colon carcinoma SW480 cell line under hypoxia conditions.

目的构建靶向缺氧诱导因子-1α特异性短发卡RNA(shRNA)的真核表达载体,为结肠癌的靶向治疗奠定基础。

Objective: To construct short-hairpin RNA expressing vectors aimed at nuclear receptor-related factor 1 (Nurrl) and study the function of Nurrl with RNA interference technology.

目的:构建针对小鼠核受体相关因子1(Nurrl)的短发夹RNA(shRNA)表达载体,为进一步体内外研究Nurrl基因的功能奠定基础。