single factor experiment

Single factor testing was used to determine the optimal concentrations of DNA template,primer,dNTP,Mg2+ and Taq DNA polymerase for PCR amplification of inter-simple sequence repeats(ISSR)in order to establish a stable ISSR-based molecular marker system for Agaricus blazei Murrill.

为了建立稳定的姬松茸(Agaricus blazei Murill)简单序列重复区间(Inter-Simple Sequence Repeats,ISSR)分子标记技术体系,笔者通过单因子试验分别研究了模板DNA、Mg2+浓度、dNTP、引物浓度和Taq酶用量对姬松茸ISSR-PCR扩增的影响,确定了姬松茸ISSR分析的最佳PCR条件为:25μL反应体系中,模板DNA20ng,引物0。

Using analytical technigue of single factor test statistics, a suitable medium and shaking culture conditions for Grifold frondosa have been selected, The experimental results showed The best culture determined is 50g/L of cornmeal, 20g/L of wheat bran respectirely, 1g/L of KH2PO4 , 0.

通过单因子试验优化筛选了适于灰树花的液体培养基和摇瓶培养条件,结果表明,适宜的培养基组成为:玉米粉50g/L、皮20g/L、KH2PO41g/L、MgSO40。

Using analytical technic of single factor test statistis,a suitable medium and shaking culture conditions for Penicillium chrysogenum Thom have been selected.

通过单因子试验统计分析 ,优化筛选了PenicilliumchrysogemumThom的适宜培养基和摇瓶培养条件 ,结果表明 ,其适宜的液体培养基中C/N比在 3 3— 2时对其孢子的萌发 ,菌丝体的生长均有

In this work,single factor experiment and response surface methodology were successfully applied to optimize the operational parameters which affected the extraction yields of ganoderic acids from the mycelia of Ganoderma lucidum.

利用单因子试验和响应面法优化了影响灵芝菌丝体灵芝酸的提取过程。

The effect of five factors(Taq DNA polymerase,Mg2+,dNTP,Primer,template DNA)on ISSR-PCR application system have been investigated with single factor tests and orthogonal design for Magnolia grandiflora.

结果表明:正交设计采用直观分析法获得影响因素最佳反应水平与单因子试验找出影响因素最佳反应水平有一定差异,通过综合比较分析,最终建立了广玉兰ISSR-PCR的最佳反应体系:在20μl的反应体系中,TaqDNA聚合酶1。

Analysis of Location and Dispersion Effects in Unreplicated Factorial Experiments with Censored Data;

带有截尾数据的无重复因子试验的位置效应与散度效应分析

Analyzing Location and Dispersion Effects from Replicated Factorial Experiments with Right-censored Data;

带有右截尾数据的有重复因子试验的数据分析

For the location effects and dispersion effects model with censored data in unreplicated factorial experiments,the new algorithm which combine model selection with simultaneous estimation of location and dipersion effects into an iterative procedure is prsented.

考虑带有截尾数据的位置效应和散度效应模型,在改进Hamada和Wu(1991)位置效应分析方法的基础上,将Brennerman和Nair(2001)散度效应分析的MH方法融入其中,对带有截尾数据的无重复因子试验给出了模型选择以及同时估计位置效应和散度效应的迭代算法,拓展了Hamada和Wu的方法,使其也可以鉴别和估计散度效应。

Study on impact factors of Quercus wutaishanica seed germination in Ziwu Mountains

子午岭辽东栎种子萌发影响因子试验研究

Static Factorial Experiment and Initial Pilot-scale Test on Bio-desulfurization of Biogas;

沼气生物脱硫静态因子试验与初步中试研究

Analyzing Location and Dispersion Effects from Replicated Factorial Experiments with Right-censored Data;

带有右截尾数据的有重复因子试验的数据分析

Influential Factorial Experiment and Index Optimization of the Soy Sauce Koji Enzyme Activity

影响酱油曲料酶活的因子试验及参数优化

Cilico Cloning and Verification of eIF4A Gene from Laver Porphyra yezoensis

条斑紫菜eIF4A基因的电子克隆与试验验证

Gene Expression and Its Clinical Research of Insulin-Like Growth Factor-1;

胰岛素样生长因子-1基因表达及临床试验研究

The Effect of Metal lons and EDTA on the Cobra Venom Factor Hemolysis Test

金属离子及EDTA对蛇毒因子溶血试验的影响

Study on Effect of Hydrometeorological Element on Algal Growth;

水文、气象因子对藻类生长影响作用的试验研究

An Experimental Research on Testing Method of a Solid State Polymer Loss Factor;

一种固态聚合物损耗因子测试方法实验研究

Experiment Design with Signal Factor and Its Application in Determination of Arsernic;

带有信号因子的试验设计及在测砷中的应用

Electric Field Assisting Low-Energy Ion Beam Mediated Gene Transfer

电场辅助离子束介导基因转化试验研究

Study on the Effect of Environmental Factors on the Photosynthesis of Elodea nuttallii

环境因子对伊乐藻光合作用影响的试验研究

Design and Analysis for Low Voltage Apparatus Multiple Factor Reliability Experiment

低压电器多因子可靠性试验设计和分析

Induction mutation of mustard gas at tk locus in L5178Y cells

L5178Y细胞tk基因突变试验评价芥子气致突性

Screening of the Key Factors of Increasing Crop Production by Orthogonal Design

正交试验法筛选风砂土上作物增产的关键因子

Application of Orthogonal Design in Establishing Sterile Rapid Propagation System of Mulberry

利用多因子正交试验建立桑的无菌快繁体系

Study on Root Induction Culture of Cymbidium by Applying Orthogonal Design

寒兰组培苗生根培养的多因子正交试验研究

Prokaryotic Expression of a Fragment of Extracellular Factor from Streptococcus Suis Type 2 and Determination of Its Immunoprotection Potency;

猪链球菌2型胞外因子基因片段的原核表达及免疫保护力试验