subculture

Exploration on Living Passage for Trichinella Isolates;

旋毛虫活体传代技术的研究

Cultivation,proliferation,and passage of human adipose tissue-derived stromal cells in vitro;

人脂肪基质细胞的分离、培养、增殖及传代稳定性

Isolation,passage and identification of HHV-6 co-infected with HIV-1;

与HIV-1共感染的HHV-6毒株的分离、传代培养与鉴定

Influence on Phenotype and Ribosomal DNA Sequence of Trichophytin rubrum Resulting from Subculture;

传代对红色毛癣菌表型和核糖体基因影响的研究

Research on Subculture and Storage Method and Genetic Analysis of Lactic Acid Bacteria Separated From DVS;

DVS乳酸菌种菌株传代和保存与遗传稳定性分析

Genetic Stability during Subculture of BCG for Vaccine Production in China

我国生产用卡介菌的传代稳定性

As well,DNA sequences of the hsBLyS gene shows no differences among the generations.

将构建的含有pET30a/hsBLyS重组质粒的BL21 (DE3)菌种,在含硫酸卡那霉素的平板培养基划线连续传代至 100代 100代次菌种经显微观察,为典型的革兰氏阴性大肠杆菌,生化特性没有改变 提取0、50、100代质粒DNA限制性内切酶切割检查,酶切图谱没有改变,DNA测序未见hsBLyS基因变异 传代前后菌种诱导表达hsBLyS,表达水平和hsBLyS生物学和免疫学活性均无明显差

The growth characteristics and the morphology of the cultures did not show differences among the generations.

将构建好的含有人变异tPA基因的重组pQE30tPAr质粒的JM10 9菌株原始种子库菌种 ,在含Amp的平板培养基划线法连续传代至 10 0代 ,其在固体培养基中生长速度、菌落形态和菌体形态、Amp抗性等方面与原始种子库无明显差异 ;每隔 10代提取质粒DNA用HindⅢ和KpnⅠ限制性内切酶切割检查 ,酶切图谱没有改变 ;DNA测序未见tPAr基因变异 。

Observation on the Stability of the Replication and Generation of HBV Gene in Transgenic Mice;

乙型肝炎病毒转基因小鼠外源基因的复制和传代稳定性观察

Results:Two high expressing founders were obtained,and have stably genetics to eighth generation by backcrosses with the average passage rate of 44 67% among offspring per genera.

3copy)转基因 Balb/ c小鼠 Founder 2只 ,用回交传代方法 ,本 HBV转基因小鼠已稳定传至第 8代 ,每代转基因鼠阳性率平均保持在 4 4 。

The cultured and transfer of cultured cells were inspected with immunocytochemistry test.

方法取孕16～18d的孕鼠,取胎鼠的嗅球,用含10%FCS的培养基培养传代分离出的细胞,免疫细胞化学方法鉴定培养和传代出的细胞,对染色阳性的细胞计数检测纯度。

The result showed the hereditary stability was fine when continuous transfer of culture of strain Q26 reached 30 generations from one tube to anther and it fitted to be used as Direct Vat Set.

为制备发酵性能优良的直投式酸奶发酵剂,研究乳酸菌Q26菌株传代过程中在转管不同次数时的形态变化,采用全菌体蛋白电泳方法对比Q26菌株转管不同次数的蛋白表达差异。

Then,the epithelial cells were purified by several differential passages and identified by immunocytochemical staining of cytokeratin14 and vimentin.

方法:分离出生后7dSD大鼠上下颌第一、二磨牙牙胚,切取牙冠颈部组织,酶消化法原代培养,利用差速传代纯化上皮细胞。

Then,the epithelial cells were purified by several differential passages and identified by immunohistochemical staining of cytokeratin14 and vimentin.

方法分离出生后3dSD大鼠下颌切牙胚,切取根尖唇侧部分组织,酶消化法原代培养,利用差速传代纯化上皮细胞。

continuous passage culture

连续传代培养（物）

Exact Borel Subalgebras and Principal Subalgebras of Quasi-hereditary Algebras;

拟遗传代数的Exact Borel子代数与主子代数

hereditary metabolic diseases of nervous system

神经系统遗传代谢病

These traditions have endured throughout the ages.

这些传统世代相传至今.

Fine traditions should be passed on from generation to generation.

优良传统要世代相传。

Capable of being passed from one generation to the next; hereditary.

可遗传的，遗传的能由一代传给下一代的；遗传的

something that has been in a family for generations.

一个家族代代相传之物。

the story was passed down from father to son.

故事被一代代流传下来。

Proceeding by descent from an ancestor.

代代相传的从祖先往下传的

The legend passed down from age to age.

这个传说一代一代地传下去。

This legend has been handed down from father to son.

这个传统是一代一代流传下来的。

This valuable heirloom has been passed down from generation to generation.

这个珍贵的传家宝是代代相传的。

To transmit(characteristics) from one generation to another.

使遗传把（特点）从一代向另一代传递

Ancient Drama Dissemination and Modern Media Art

古代戏剧的传播美学与现代传播艺术

A tradition must be capable of being passed down from one generation to another.

传统必须能一代一代往下传，不能传的，还能叫“传统”吗？

A lie begets a lie till they come to generation

谎言生谎言,谎言传万代

The legend springs from ancient China.

这传说来自古代的中国。

intergenerational cycle of poverty

世代相传的贫穷循环