dna joinase

A thermostable DNA ligase gene was identified, and the biochemical and enzymatic properties of the ligase were characterized from JP2 strain which was enriched from geothermally active sites in Papua New Guinea.

对分离自巴布亚新几内亚地热活跃区的一种嗜热耐酸古细菌——JP2菌株中的DNA连接酶基因进行了克隆、表达、纯化,并对其生物化学及酶学特性进行了研究。

DNA ligase from the hyperthermophilic crenarcheon Sulfolobus shibatae (Ssh ligase) was optimally active in the presence of ATP and partially active in the presence of dATP.

极端嗜热古菌——芝田硫化叶菌DNA连接酶(Ssh连接酶) 的最适辅因子为ATP,在dATP存在时,该酶也能表现出较弱的连接活性。

Two genes encoding a NAD+-dependent DNA ligase (LigA) and an ATP-dependent DNA ligase (LigB) were identified in the genome of the extremely radioresistant bacterium.

在耐辐射球菌基因组中有两个基因分别编码DNA连接酶,一个是NAD~+依赖型DNA连接酶(LigA),一个是ATP依赖型DNA连接酶(LigB)。

TEB4(MARCH VI)is the homolog of yeast Doa10,an E3 ligase involved in ER-associated degradation.

TEB4(MARCH Ⅳ)是酵母Doa10的同源体,作为E3连接酶参与内质网相关蛋白的降解。

Modulating effect of androgen on ubiquitin ligases in a rat model of skeletal muscle atrophy;

雄激素对骨骼肌萎缩大鼠泛素连接酶的调控作用研究

In this work, by using specifity, real-time and simplicity of molecular beacons and coenzyme-dependence of DNA ligase, novel detection methods for adenosine triphosphate (ATP), nicotinamide adenine dinucleotide phosphate (NADP), creatine kinase (CK) and protein kinase A (PKA) were developed:1.

本论文基于分子信标技术实时、快速、简便的特点,结合连接酶对辅酶的高度依赖性,以三磷酸腺苷(ATP)、烟酰胺腺嘌呤二核苷酸磷酸(NADP)、肌酸激酶(CK)、蛋白激酶A(PKA)为目标,着眼于发展生物分子检测的新方法,开展了以下四个工作:1。