pestilent toxicity

The propagation characteristics of virulent duck plague virus(DPV)in duck embryo fibroblast(DEF)were studied by the method of light microscopy observation of DEF cell culture monolayer,electron microscopy observation of infected DEF cell culture,real-time PCR detecting virus propagation.

通过细胞培养物光学显微观察、细胞超薄切片研究、定量PCR检测技术对鸭胚成纤维细胞中鸭瘟强毒的增殖特性进行了研究。

Expression and Immunological Activity of E2 Epitope of Classical swine fever virus;

猪瘟病毒E2蛋白抗原表位的表达与免疫活性研究

Pathogenicity of classical swine fever virus GX-3/98 strain and immunization relativity with attenuated hogcholera virus Chinese C strain;

猪瘟病毒广西地方流行株GX-3/98的致病性及其与兔化弱毒株的免疫相关性

Kinetics of peripheral blood leukocytes apoptosis and changes of CD4~+ and CD8~+ T lymphocyte subpopulations of pigs infected with classical swine fever virus;

猪瘟病毒感染猪外周血白细胞凋亡及CD4~+和CD8~+T淋巴细胞亚群的变化

The recombinant retroviral vector pBABE-puro-E2 was constructed by inserting classical swine fever virus(CSFV) Shimen strain E2 gene into pBABE-puro.

利用DNA重组技术将猪瘟病毒石门株囊膜蛋白E2基因定向插入逆转录病毒载体pBABE-puro中,构建重组逆转录病毒载体pBABE-puro-E2。

A classical swine fever virus(CSFV) E2 gene was codon-optimized according to the Sf9 cell codon usage.

根据昆虫细胞密码子偏嗜性,对猪瘟病毒E2基因进行密码子优化,并利用昆虫杆状病毒表达系统进行了表达,表达水平约为野生型E2基因的3倍。

A truncated gene encoding the major antigenic domains of E2 protein of classical swine fever virus(CSFV) was amplified by RT-PCR from the genomic RNA of CSFV C-strain and cloned into pPROEX-HTb expression vector to obtain recombinant pPROEX-tE2.

利用RT-PCR扩增了猪瘟病毒(CSFV)兔化弱毒疫苗株(C株)的E2蛋白主要抗原区(tE2)编码区,并定向克隆到表达载体pPROEX-HTb中,获得重组表达载体pPROEX-tE2,将其转化大肠杆菌DH5α菌株,经IPTG诱导,tE2蛋白获得高效表达;经检测,该重组蛋白能被CSFV C株抗血清识别。

Fusion expression of multiepitope gene of CSFV envelope glycoprotein E2 and its protection efficiency against homologous challenge in rabbits;

猪瘟病毒E2蛋白重复多表位基因的融合表达及其兔体免疫保护研究

Prediction,synthesis and characteristics of B-cell epitopes of CSFV envelope glycoprotein E2;

猪瘟病毒E2糖蛋白抗原表位的预测、多肽合成及特性研究

Detection of the Interference to the Reproduction of CSFV by PK-15 Cell Strains Transcribing shRNA Targeted to the 3′-UTR;

转录靶向猪瘟病毒3′-UTRshRNA细胞株干扰猪瘟病毒增殖的检测

Dynamics of IFN-α mRNA expression in liver of ducks infected with duck plague virus of different virulence;

鸭瘟病毒疫苗株与强毒株诱导雏鸭IFN-α mRNA在肝脏中表达的动态定量研究

Purification and electron microscopy observation after negative staining of duck plague virus;

鸭瘟病毒的纯化及电镜负染形态观察

Prokaryotic expression of N-terminal antigenic domain of duck plague virus gB protein and the establishment of putative indirect ELISA assay;

鸭瘟病毒gB蛋白N端主要抗原域的表达及间接ELISA检测

Prokaryotic Expression of TK Gene from Virulent Duck Plague Virus and Its Cellular Localization in Infected Duck Tissue;

鸭瘟强毒TK基因的原核表达及在感染鸭组织亚细胞定位研究

DPV dUTPase Gene Prokaryotic Expression and the Study of Its Subcellular Localization in Virus-infected Host Cells;

鸭瘟强毒dUTPase基因的原核表达及在病毒感染宿主亚细胞定位研究

Study on Dynamic Distribution in Ducks and the Ablity to Anti Duck Plague Virus with Expressive Product in Vitro of DuIFN-α Gene Vaccine;

鸭α-干扰素核酸疫苗在鸭体内的动态分布及表达产物抗鸭瘟强毒效果初探

Studies on Large-scale Production of pcDNA-SDIFN-α Vaccine by Fermentation and pcDNA-SDIFN-α Inmmunized Ducks Against Challenge Infection of Duck Plague Virus;

鸭α-干扰素基因疫苗高密度发酵及免疫鸭抗鸭瘟强毒人工感染的研究

Morphogenesis and Ultrastructural Studies in DPV Artificially Infected Ducks and the Discovering and Preliminary Studying of DPV dUTPase Gene;

鸭瘟强毒在实验感染鸭体内的致病与复制特征及dUTPase基因发现和初步鉴定

Studies on Dynamic Distribution, Mucosal Immunity and Influence for Intestinal Microbial Community of Ducks Infected with Duck Plague Virus Virulent Strain;

家鸭人工感染鸭瘟强毒株的动态分布、黏膜免疫及对肠道菌群结构的影响

Analysis of TK gene of a virulent duck plague virus CY07 strain

鸭瘟病毒CY07强毒株TK基因的序列分析

Mucosal and Systemic Immune Response to the Distribution of Virulent or Attenuated Duck Plague Virus in Inoculated Ducklings;

鸭瘟强、弱毒株在感染鸭体内的动态分布及免疫应答

Studies on the Detetion of Antibodies Against New Duck Plague Virus by an Indirect ELISA and the Detection of New Duck Plague Virus by an Indirect Immunofluorescent Assay;

间接ELISA检测新型鸭瘟抗体和IFA检测新型鸭瘟病毒的研究

Development and Verification of PCR for Detection of Duck Plague Virus

鸭瘟病毒PCR检测方法的建立及验证

Cloning and Molecular Characterization of VP26 Gene of Duck Plauge Virus

鸭瘟病毒VP26基因克隆和分子特性分析

Analysis of molecular characteristics of duck plague virus UL24 gene

鸭瘟病毒UL24基因的分子特征分析

Development of a multiplex PCR assay for detection of goose parvovirus and duck plague virus

鹅细小病毒和鸭瘟病毒复合PCR检测方法的建立

Duck Hemorrhagic Disease(DHD)is a new infectious disease caused by a new type herpesivrus diferent from duck plague virus.

鸭出血症是由不同于鸭瘟病毒的鸭新型疱疹病毒引起的鸭的一种传染性疾病。

Discovery, Prokaryotic Expression and Applications of Duck Plague Virus gC Gene;

鸭瘟病毒gC基因的发现、原核表达和应用研究

Studies on the Discovery, Prokaryotic Expression and Applications of Duck Plague Virus US3 Gene

鸭瘟病毒US3基因的发现、原核表达及应用研究

DPV UL25 Gene Prokaryotic Expression and the Study of Its Cellular Localization in Virus-infected Cells

鸭瘟病毒UL25基因的原核表达及细胞定位研究

Transient expression of duck plague virus UL51 gene in COS-7 cells

鸭瘟病毒UL51基因在COS-7细胞中的瞬时表达