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Construction and identification of a vector expressing short hairpin RNA targeting annexinⅡgene;

膜联蛋白Ⅱ基因短发夹RNA表达载体的构建和鉴定

The effect of short hairpin RNA on hTERT expression;

短发夹RNA对人端粒酶催化亚基表达的影响

Objective To clone shRNA (short hairpin RNA) recombinant plasmid targeting on COX-2 gene and analyze the nucleic acid sequence of the recombinant plasmid.

目的利用RNA干扰技术,以COX-2为靶基因,构建靶向COX-2基因的短发夹RNA(shRNA)重组表达质粒并进行鉴定分析。

Inhibition of ICAM-1 gene expression in liver cancer cells by shRNA;

短发夹RNA对肝癌细胞细胞间粘附分子-1基因表达的影响

Objectives To investigate nano-carrier G9 PAMAM mediated plasmid containing CTGF-shRNA (pCTGF-shRNA) transfection on connective tissue growth factor (CTGF) expression in mouse peritoneal mesothelial cells (MPMCs).

目的研究G9PAMAM纳米载体介导的结缔组织生长因子(CTGF)短发夹RNA(shRNA)质粒(pCTGF-shRNA)对小鼠腹膜间皮细胞CTGF表达的影响。

The annealed oligonucleotides were inserted into the linearized pSIREN-RetroQ vector to construct the recombinant shRNA plasmid(pSIREN-VEGF-C) and then transformed into E.

方法:根据GenBank中VEGF-C序列,设计、合成靶向VEGF-C基因、编码短发夹RNA的两条寡核苷酸序列,退火后用T4连接酶与线性化的pSIREN载体连接,转化感受态大肠杆菌,筛选阳性克隆,抽提质粒,获得重组pSIREN-VEGF-C质粒;BglⅡ和EcoRⅠ双酶切、测序鉴定。

Objective: To transfect a recombinant short hairpin RNA(shRNA)expression vector targeting Livin gene isoform(BIRC71,BIRC72)into the cervical cancer cell line(Hela cell),in an attempt to observe RNAi-mediated silen- cing on Livin gene and the induction of Hela apoptosis.

目的:应用构建成功的Livin异构体(BIRC71,BIRC72)短发夹RNA(short hairpin HNA,shRNA)真核表达载体转染Hela细胞,探讨其对Livin基因的沉默效应及诱导Hela细胞凋亡的作用。

Objective: To construct short-hairpin RNA expressing vectors aimed at nuclear receptor-related factor 1 (Nurrl) and study the function of Nurrl with RNA interference technology.

目的:构建针对小鼠核受体相关因子1(Nurrl)的短发夹RNA(shRNA)表达载体,为进一步体内外研究Nurrl基因的功能奠定基础。

Objective To construct effective short-hairpin RNA (shRNA) expression vector encoding shRNA targeting LRIG2 gene, and to screen the stably transfected cell clone.

目的构建针对富含亮氨酸重复序列免疫球蛋白样蛋白2(LRIG2)基因的短发夹RNA(shRNA)表达载体,建立稳定转染的细胞株,观察其对目的基因表达的影响。

Construction of the shRNA expression vectors targeting survivin

Survivin靶向的短发夹RNA表达载体的构建

The Studies on Depressant Effect of Short-Hairpin RNA of Bcl-xL Gene Expression in HepG-2 Cells;

短发夹RNA抑制人肝癌HepG-2细胞Bcl-xL基因表达的探讨

The Studies on Interference Effect of Short Hairpin RNA on Expression of TGF-β1 Gene in Human Lung Fibroblast;

短发夹RNA抑制人肺成纤维细胞TGF-β1基因表达的研究

RNA interfering effects of CCL20 shRNA expressing vectors on human embryonic kidney cells

CCL20基因短发夹RNA表达载体转染人胚胎肾细胞的干扰效应

Inhibitory effect of β-catenin short hairpin RNA on the growth of human colon cancer xenografts in nude mice

靶向β-catenin短发夹RNA抑制人结肠癌裸鼠移植瘤的实验研究

Silence Effect of Short Hairpin RNA on Expression of PIM-1 in Prostate Cancer Cell Lines

短发夹RNA对前列腺癌细胞中PIM-1基因沉默效应的研究

Construction of LRIG2 Specific Short-hairpin RNA Expressing Vector and Screening of Stably Transfected Cell Clone

LRIG2基因短发夹RNA表达载体的构建、鉴定和稳定株的筛选

Inhibitory effect of short hairpin RNA targeting IRAK-M gene for endotoxin tolerance in RAW264.7 cells

IRAK-M短发夹RNA对RAW264.7细胞内毒素耐受性的抑制作用

RNA interference targeting hypoxia inducible factor-1α in BGC-823 cells inhibits cell proliferation and induces cell apoptosis

短发夹RNA干扰胃癌低氧诱导因子-1α表达及其临床意义

Gene Silencing by Systemic Delivery of Adenovirus Vector-mediated Short Hairpin RNA of Rat Angiotensin Receptor Subtype 1 in Spontaneously Hypertensive Rats;

腺病毒介导的大鼠血管紧张素Ⅱ1型受体短发夹RNA在SHR的基因沉默作用研究

Effect of Suppressing COX-2 Expression by shRNA on Adhesion and Invasion of Human Hepatocellular Carcinoma Cell;

短发夹状RNA抑制COX-2表达对人肝癌细胞株黏附侵袭力的影响

The Research about Interference of Nucleostemin-Specific Short Hairpin RNA in Vitro and in Vivo;

核干细胞因子特异性短发夹状RNA的体内外干扰作用研究

Construction and Identification of Multidrug Resistance Gene MDR1 Short Hairpin Expression Plasmids;

多药耐药基因MDR1短发夹状RNA表达质粒的构建与鉴定

Mechanism of Short Hairpin RNA Silence NF-κB Promotion Apoptosis of Hepatic Stellate Cell;

应用短发夹状RNA沉默NF-κB基因促进肝星状细胞凋亡机制研究

The Effect of Short Hairpin RNA of Nuclear Factor Kappa B on the Apoptosis of Hepatic Stellate Cell;

核因子-κB亚单位p65短发夹状干扰RNA对肝星状细胞凋亡的影响

Inhibition of Bcl-2 by shRNA to Induce Apoptosis in Human Lens Epithelial Cells

短发夹状RNA干扰Bcl-2诱导晶状体上皮细胞凋亡的实验研究

Construction and identification of lentiviral vector-mediated short hairpin RNA of human EGFL7 gene

人EGFL7基因短发夹结构RNA慢病毒载体的构建及鉴定

Reversal of Leukemia Multidrug Resistance by Sequence-Specific Short Hairpin RNA

序列特异性短发夹状RNA逆转白血病多药耐药性研究